Recognizes native and denatured-reduced forms of synthetic poly-histidine or poly-histidine-tagged fusion proteins. Recognizes fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.
Usage: Suitable for use in Western Blot, ELISA and Immunohistochemistry. Western Blot: 1:10000. ELISA: 1:10000-1:50000 using 50-100 ng control antigen/well. Immunohistochemistry: 1:200-1:2000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
0.05 % merthiolate
Agent conservateur
Thimerosal (Merthiolate)
Précaution d'utilisation
This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
avoid freeze thaw cycles.
Stock
4 °C,-20 °C
Stockage commentaire
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.